The enzyme-linked immunosorbent assay (ELISA) was independently developed in 1971 by Engall and Perlmann and by van Weemen and Schuurs with the aim of avoiding the problems associated with radioimmunoassays. Since then, ELISA has become a widely used method for detecting polyclonal and monoclonal antibodies in biological fluids or culture media.
Offering advantages such as high specificity,...
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